This laboratory module, published on CourseSource, leads introductory biology students in the exploration of a basic set of bioinformatics concepts and tools.

The purpose of this semester-long Lesson is to give students an authentic, course-based undergraduate research experience during which they learn basic and advanced microbiological and molecular biology techniques. This project begins with the isolation of a suspected Streptomyces bacterium from a soil sample and concludes with its identification. Students collect data, regarding colony and cell morphology, biochemical characteristics, the production of secondary metabolites, and employs the PCR using custom-designed primers to the Streptomyces 16s rRNA gene. The project culminates with the identification of their soil isolate using the National Center for Biotechnology Information (NCBI) web site to perform nucleotide blasts. The blastn program provides the final piece of evidence used to confirm, or not, the identification of their isolate as a Streptomyces from 16s rRNA gene sequence data, hence the title “From Dirt to Streptomyces DNA. In addition, the Lesson focuses on the Streptomyces bacteria to address several ASM aligned goals and objectives. These include prokaryotic growth phases and ways in which interactions of microorganisms among themselves and with their environment is determined by their metabolic abilities.  In addition, this Lesson illustrates how microbial metabolism is important to a relevant societal issue, the need for new antibiotic discovery particularly given the rise of antibiotic resistance strains of clinically relevant bacteria. It also illustrates the microbial diversity of soil and the developmental/physiological strategies employed in such a competitive environment. This Lesson hopes to impart both the thrill and challenges associated with scientific discovery.

Primary image: Photomicrograph of Streptomyces colonies growing on ISP 2 agar. The Streptomyces are student isolates showing stages of morphological development. Photomicrograph by Marc A. Brodkin.

This lesson focuses on urbanization and its negative effects on species, specifically amphibians. The lesson will also provide hands-on statistical analyses and critical thinking questions to promote a better understanding of this ecological problem.

Dr. Christopher Schell is an ecologist who uses a variety of techniques to study mammalian carnivores in urban areas. This scientist spotlight uses graphs from his study on parental habituation to human disturbance in coyotes.

Presented at NABT 2019, we demonstrate how the Population Dynamics Click & Learn resource can be customized for any organism - using the Lionfish invasion as an example

This web page contains materials created by faculty of the University of Nebraska-Lincoln Department of Mathematics to teach basic fundamentals of mathematical epidemiology.

Rapid advances in genomics and bioinformatics, the vast amount of data generated by next-generation sequencing, and the penetration of the ‘-omics’ into many areas of biology have created a need for students with hands-on experience with computational and ‘big data’ methods. Additionally, laboratory experience in the isolation, identification, and characterization of unknown bacteria is a vital part of a microbiology student’s training. This lesson is a course-based undergraduate research experience (CURE) focusing on Salmonella enterica, a common and relatively low-virulence foodborne pathogen. In Module 1, students isolate and identify S. enterica strains from stream sediment, poultry litter, or other sources. They conduct phenotypic evaluation of antimicrobial resistance (AMR) and can search for plasmids. Isolates’ whole genomes may be sequenced by the United States FDA or public health laboratories, typically at no charge. In Module 2, students learn basic methods of genome assembly, analysis, annotation, and comparative genomics. They use easily accessible, primarily web-based tools to assemble their genomes and investigate areas of interest including serotype, AMR genes, and in silico evidence of mobile genetic elements. Either module can be used as a standalone learning experience. After course completion, students will be able to isolate and identify Salmonella from natural sources, and use computational analysis of microbial genomic data, particularly of the Enterobacteriaceae. This lesson offers undergraduate microbiologists a genuine research experience and a real-world microbiology application in genomic epidemiology, as well as a valuable mix of field, laboratory, and computational skills and experiences.

Remote learning often requires an alternative to hands-on, student-designed research projects. To this end, we created a package of scaffolded assignments to support introductory students through the research process using datasets from past student projects. These assignments provide opportunities for active practice and feedback on skills in experimental design, data analysis, literature review, and scientific communication. While we created the assignments to be heavily guided and focused on organismal biology to support students in our particular course context, the documents are highly customizable to meet the learning objectives for other course formats, subjects, and levels.

This module contains information for a three part series introducing the venom system in reptiles and discussing it in an evolutionary context. In the first part venom and its ecological roles are defined with a discussion of the diversity of venom structures and venomous lineages, primarily in squamates. Examples are provided of the various ways that venoms may vary among biological scales. In parts 2 and 3, the evolution of venom is discussed. Part 2 focuses on a description of how the venom system arose in squamates and a discussion of the challenges associated with defining "venomousness". Part 3 examines the various genetic mechanisms that produce venom variation using examples from primarily literature that are presented in Part 1. In addition to lecture materials, we include a primary literature based activity and a group activity designed to encourage students to explore the diversity of venomous taxa in reptiles and amphibians.